Table 2.

Primer sequence and location

Gene and GenBank accession #	Location in cDNA	Primer sequence (actual sequence)
hGAPDH # M17851	outer primers: bp 618-647 bp 997-968 inner primers: bp 681-710 bp 1000-971 inner-inner primers: bp 617-646 bp 936-907	5’-CATCCCTGCCTCTACTGGCGCTGCCAAGGC-3’ 5’-AGGCCATGAGGGCCATGAGGTCCACCACCC-3’ 5’-TGTGGGCAAGGTCATCCCTGAGCTG AACGG-3’ 5’-TGTTGCTGTAGCCAAATTCGTTGTCATACC-3’ 5’-GAAGCTCACTGGCATGGCCTTCCGTGTCCC-3’ 5’-AGGAAATGAGCTTGACAAAGTGGTCGTTGA-3’
hRORγT #U16997	outer primers: bp 1231-1260 bp 1590-1561 inner primers: bp 1262-1291 bp 1560-1531 inner-inner primers: bp 1293-1322 bp 1526-1495	5'-TTCGAGCCTTGGGCTGCAGCGAG CTCATC-3' 5'-GCTGAAGAGCTCCTTGTAGAGTGGAGGGAA-3' 5'-GCTCCATCTTTGACTTCTCCCACTCCCTAA-3' 5'-AGCGGCTTGGACCACGATGGGGTGGAGGTC-3' 5'-TGCCTTGCATTTTCCGAGGA TGAGATTGC-3' 5'-AAGATCTGCAGCCTTTCCACATGC TGGCTACA-3'
hCD70 # BC000725)	outer primers: bp 301-330 bp 660-631 inner primers: bp 331-360 bp 630-601 inner-inner primers: bp 361-390 bp 600-571	5’-CAGGACCCCAGGCTATACTGGCAGGGGGGC-3’ 5’-CACTCCAAAGAAGGTCTCATCAGTGTTTCG-3’ 5’-CCAGCACTGGGCCGCTCCTTCCTGCATGGA-3’ 5’-GGAAGGCAAAAGTGTCCCAGTGAGGTTGGT-3’ 5’-CCAGAGCTGGACAAGGGGCAGCTACGTATC-3’ 5’-GCAGAGTGTGTCCCCTCGGGCCAGGGGCGT-3’
HCD27 # NM_001242	outer primers: bp 541-570 bp 990-961 inner primers: bp 571-600 bp 960-931 inner-inner primers: bp 601-630 bp 930-901	5’-GCAGTGCAGGGACAAGGAGTGCACCGAGTG-3’ 5’-GGGGAGCAGGCAGGCTCCGGTTTTCGGTAA-3’ 5’-TGATCCTCTTCCAAACCCTTCGCTGACCGC-3’ 5’-CTTCCCTGGATGGGGATGGAGCTGCCCTCC-3’ 5’-TCGGTCGTCTCAGGCCCTGAGCCCACACCC-3’ 5’-TCCTCCCTGGGGCAGCTGTAACGACAAGGC-3’
hIL-17A # NM_002190	outer primers: bp 361-390 bp 780-750 inner primers: bp 391-420 bp 750-721 inner-inner primers bp 421-450 bp 721-691	5’-GTGGACTACCACATGAACTCTGTCCCCATC-3’ 5’-CATAAATAACTTAAAGCCCTTAAAAAAGTA-3’ 5’-CAGCAAGAGATCCTGGTCCTGCGCAGGGAG-3’ 5’-AACTTGAAGCAAATTGGTACTCAGTCAAAC-3’ 5’-CCTCCACACTGCCCCAACTCCTTCCGGCTG-3’ 5’-CTTCCTTCTTGGAAAGAGGGAAAGGTAATT-3’
hIL-17B # NM_014443	outer primers: bp 121-150 bp 590-561 inner primers: bp 391-420 bp 560-531 inner-inner primers: bp181-210 bp 530-501	5’-GCCCCAAAAGCAAGAGGAAGGGGCAAGGGC-3’ 5’-GAAGATGCAGGAGCAGCCCACAGCGATGGT-3’ 5’-GGCCTGGGCCCCTGGCCCCTGGCCCTCACC-3’ 5’-CTCCATGACTGCGCGCTGGCGGCAAGGCCC-3’ 5’-AGGTGCCACTGGACCTGGTGTCACGGATGA-3’ 5’-TGTGCGGGGCGGTGGCGGGCAGAGGCGGCG-3’
hIL-17C # NM_013278	outer primers: bp 241-270 bp 640-611 inner primers: bp271-300 bp 610-581 inner-inner primers: bp 301-330 bp 580-551	5’-AGCCCTGGTGTCCAGCCTGGAGGCAGCAAG-3’ 5’-CACTGAACGGGGCAGCACGCAGGTGCAGCC-3’ 5’-CCACAGGGGGAGGCACGAGAGGCCCTCAGC-3’ 5’-ACGGGGACGTGGATGAACTCGGTGTGGAA-3’ 5’-TACGACCCAGTGCCCGGTGCTGCGGCCGGA-3’ 5’-GGCAAAGGCCCCAGGTGTGGGGAGCCCCGA-3’
hIL-17D # NM_138284	outer primers: bp 241-270 bp 810-781 inner primers: bp 271-300 bp 780-751 inner-inner primers: bp 301-339 bp 750-721	5’-CGCCTGGCGGCCGGCGTGCTCAGTGCCTTC-3’ 5’-CCAGCGGGCGCGTCGTTGGGGCCCAGCAGG-3’ 5’-CACCACACGCTGCAGCTGGGGCCGCGTGAG-3’ 5’-AGCTTGGCGCCCTGTTTGTCGATGCTGGAG-3’ 5’-CAGGCGCGCAACGCGAGCTGCCCGGCAGGG-3’ 5’-TTGATGCTGTCTGCGTCCTTCTCCGGCTCG-3’
hIL-17E # NM_022789	outer primers: bp 421-450 bp 840-810 inner primers: bp 451-480 bp 810-781 inner-inner primers: bp 481-510 bp 780-751	5’-CAGCTGCTGCCCCAGCAAAGGGCAGGACAC-3’ 5’-CCCATCACACGGGGCCGCACACACACACAA-3’ 5’-CTCTGAGGAGCTGCTGAGGTGGAGCACTGT-3’ 5’-GCTAAGGAAACACGGTACAGCCTGCGCTCC-3’ 5’-GCCTGTGCCTCCCCTAGAGCCTGCTAGGCC-3’ 5’-AGGCAGTAGCCCTTGTGGGTGCCCTTCTCG-3’
hIL-17F # BC070124	outer primers: bp 81-110 bp 540-511 inner primers: bp 111-140 bp 510-481 inner-inner primers: bp 141-170 bp 480-451	5’-AAGACCCTGCATGGCCCAGCCATGGTCAAG-3’ 5’-GGGTGACGCAGGTGCAGCCAACAGTCACCA-3’ 5’-TACTTGCTGCTGTCGATATTGGGGCTTGCC-3’ 5’-GCACCTTCTCCAACTGGAAAGAAACAGAGC-3’ 5’-TTTCTGAGTGAGGCGGCAGCTCGGAAAATC-3’ 5’-AGCCTTGGTGCTTCCTCCGGACGACCAGGG-3’
HPLZF-1 # BC029812	outer primers: bp 1891-1920 bp 2220-2161 inner primers: bp 1921-1950 bp 2160-2131 inner-inner primers: bp 1951-1980 bp 2160-2131	5’-GAGTTCTGTGGCAGCTGCTTCCGGGATGAG-3’ 5’-CGGGATCTCCTCGGGCTTGTGGCCCTTCAT-3’ 5’-AGCACACTCAAGAGCCACAAACGCATCCAC-3’ 5’-GTGCTTCTGCATGGAGGAGAGGCTGGGGCA-3’ 5’-ACGGGTGAGAAACCCTACGAGTGCAATGGC-3’ 5’-GTACTCTGTGCAGATGGTGCA CTGGTAGGG-3’
HCTLA4 # AF414120	outer primers: bp 211-240 bp 480-461 inner primers: bp 245-275 bp 460-431 inner-inner primers: bp 281-306 bp 425-396	5’-CCTGGCCCTGCACTCTCCTGTTTTTTCTTC-3’ 5’-ATCCCTGTCTTCTGCAAAGCAATGCACGTG-3’ 5’-CATGTAGGTTGCCGCACAGACTTCAGTCAC-3’ 5’-ATGGAATCATCTAGGAAGG TCAACTCATTC-3’ 5’- GCCTGCTGTGGTACTGGCCAGCAGCC -3’ 5’-ACTTGATTTCCACTGGAGGTGCCCGTGCAG-3’
hFoxP3 # AF277993	outer primers: bp 961-990 bp 1440-1471 inner primers: bp 991-1020 bp 1410-1381 inner-inner primers: bp 1021-1050 bp 1380-1351	5’-CCCACCTGGCTGGGAAAATGGCACTGACCA-3’ 5’-AGGCTTCATCTGTGGCATCATCCGACAAGG-3’ 5’-GCTCCTGCTGCATCGTAGCTGCTGGCAGCC-3’ 5’-CCCGCACAAAGCACTTGTGCAGACTCAGGT-3’ 5’-CGGTCCACACAGCCCCCTTCTCGCTCTCCA-3’ 5’-TCCGTTTCTTGCGGAACTCCAGCTCATCCA-3’
hIL22 # NM_020525	outer primers: bp 121-150 bp 590-561 inner primers: bp 151-180 bp 560-531 inner-inner primers: bp 181-210 bp 530-501	5’-TCTTGGCCCTCTTGGTACAGGGAGGAGCAG-3’ 5’-AATGCAGGCATTTCTCAGAGACATAAACAG-3’ 5’- CTGCGCCCATCAGCTCCCACTGCAGGCTTG-3’ 5’-CAAATCCAGTTCTCCAATTGCTTTGATCTC-3’ 5’- ACAAGTCCAACTTCCAGCAGCCCTATATCA-3’ 5’-TCCACTCTCTCCAAGCTTTTTCACTGAGAC-3’
hiNOS2 # NM_000625.4	outer primers: bp 3121-3150 bp 3720-3691 inner primers: bp 3151-3180 bp 3690-3661 inner-inner primers: bp 3181-3210 bp 3660-3631	5’-CCCCAAGACCCAGTGCCCTGCTTTGTGCGG-3’ 5’-CGCTGACATCTCCAGGCTGCTGGGCTGCAC-3’ 5’-AATGCCAGCGGCTTCCACCTCCCCGAGGAT-3’ 5’-CGCCACCCTGTCCTTCTTCGCCTCGTAAGG-3’ 5’-CCCTCCCATCCTTGCATCCTCATCGGGCCT-3’ 5’-AAATTCTGCACCAAAGATATCTTCGTGATA-3’
hARNT # NM_001668	outer primers: bp 2101-2130 bp 2490-2461 inner primers: bp 2131-2160 bp 2460-2431 inner-innr primers: bp 2161-2190 bp 2430-2401	5’-CTACCCAGGCTACTGCTAAGACTCGTACTT-3’ 5’-TAGTTAGATCAGGGAATTCTTCATTGTTGT-3’ 5’-CCCAGTTTGGTGTGGGCAGCTTTCAGACTC-3’ 5’-AGCTGTTGCTCTGATCTCCCAGCATGGACA-3’ 5’-CATCCTCCTTCAGCTCCATGTCCCTCCCTG-3’ 5’-GCATCTCCTGGAAGACCTCAGGCTGGCCAG-3’
hIDO # NM_002164.4	outer primers: bp 871-900 bp 1320-1291 inner primers: bp 901-930 bp 1290-1261 inner-inner primers: bp 931-960 bp 1260-1231	5’-TGGGAAGACCCAAAGGAGTTTGCAGGGGGC-3’ 5’-TTCCTTCAAAAGGGATTTCTCAGTTGTACT-3’ 5’-AGTGCAGGCCAAAGCAGCGTCTTTCAGTGC-3’ 5’-TCTTACAGTCTTCAGGAAATTCATTAAATC-3’ 5’-TTTGACGTCCTGCTGGGCATCCAGCAGACT-3’ 5’-AGTGCCTCCAGTTCCTTTGGCTTCCAGTTT-3’
hGARP # Z24680.1	outer primers: bp 1591-1620 bp 2070-2041 inner primers: bp 1621-1650 bp 2040-2011 inner-inner primers: bp 1651-1680 bp 2010-1981	5’-GGGCAACGGGCTGATGGTCCTGCAGGTGGA-3’ 5’-TGTTGGTTAAACTTCTGCCGGCGGACGCAG-3’ 5’-CCTGCCCTGCTTCATCTGCCTCAAGCGGCT-3’ 5’-CAGCAGGCGGCCAGCGTGGTGCGGAGGATG-3’ 5’-CAATCTTGCCGAGAACCGCCTGAGCCACCT-3’ 5’-GCAGAGACCAGTATGAAGGTGAGGATGATG-3’
hIL9 # NM_000590	outer primers: bp 21-50 bp 440-411 inner primers: bp 51-80 bp 410-381 inner-inner primers: bp 81-110 bp 380-351	5’-GCCATGGTCCTTACCTCTGCCCTGCTCCTG-3’ 5’-CTTGCCTCTCATCCCTCTCATCTTTTCTTT-3’ 5’-TGCTCCGTGGCAGGCCAGGGGTGTCCAACC-3’ 5’-CTGGAAAATTTCCAGAAGACTCTTCAGAAA-3’ 5’-TTGGCGGGGATCCTGGACATCAACTTCCTC-3’ 5’-TGTCAGCGCGTTGCCTGCCGTGGTTTGGTT-3’
hCD46 # NM_002389.4	outer primers: bp 691-720 bp 1260-1231 inner primers: bp 721-750 bp 1230-1201 inner-inner primers: bp 751-780 bp 1200--1271	5’-ACACACCTTTAGTGAAGTAGAAGTATTTGA-3’ 5’-CAAATTACTGCAACTCCAACAACTATGGCA-3’ 5’-GTATCTTGATGCAGTAACTTATAGTTGTGA-3’ 5’-ATAACAATCACAGCAATGACCCAAACATCC-3’ 5’-TCCTGCACCTGGACCAGATCCATTTTCACT-3’ 5’-AAACTGTCAAGTATTCCTTCCTCAGGTTTA-3’
hAHR # BC070080.1	outer primers: bp 2611-2640 bp 3060-3031 inner primers: bp 2641-2670 bp 3030-3001 inner-inner primers: bp 2671-2700 bp 3000-2971	5’-ATGCCTTATACACAGAACTTTATTTCCTGT-3’ 5’-TGTCAAATCAGGAAAAGGTCTGGCTTCTGA-3’ 5’-AATCAGCCTGTATTACCACAACATTCCAAA-3’ 5’-CGGATGATGAAGTGGCTGAAGATGTGTGGT-3’ 5’-TGTACAGAGCTGGACTACCCTATGGGGAGT-3’ 5’-AGTCTGAGTGTTATTTATGTTATTTAATTC-3’