Table 5.
Quantification of Different Cholesterol-Metabolizing CYPs and Their Redox Partners in the Human Temporal Lobe, Retina, and RPEa
| Protein (pmol/mg tissue protein) | ||||||
|---|---|---|---|---|---|---|
| Mitochondria | Microsomes | |||||
| Tissue | FdR | Fdx | CYP27A1 | CPR | CYP7B1 | CYP46A1 |
| Temporal lobe | 4.35 ± 0.87 | 1.40 ± 0.25 | 0.19 ± 0.05 | 3.24 ±0.62 | 0.29 ± 0.06 | 0.40 ± 0.08 |
| Retina | 1.35 ± 0.24 | 1.37 ± 0.23 | 0.50 ± 0.07 | 2.52 ± 0.40 | 0.90 ± 0.16 | 0.10 ± 0.02 |
| RPE | 2.29 ± 0.47 | 2.38 ± 0.48 | 1.24 ± 0.40 | 2.52 ± 0.54 | 1.79 ± 0.40 | ND |
a
Proteins are grouped as mitochondrial and microsomal. Measurements were performed on tissues obtained from four human donors (Table S1). The concentration was calculated for three experimental replicates by monitoring three transitions per individual peptide for two individual peptides per protein and presented as mean ± SD. The monitored transitions are summarized in the Supporting Information (Table S2). ND, not detected.