Figure 3. Preexisting VV immunity does not diminish the control of a pathogenic intrarectal SIV challenge.

Temporal viremia (A) median for the group and (B) for individual animals. Frequency of CD4 T cells expressed as a percent of total T cells in the colorectal tissue (C) median for group and (D) for individual animals. Dotted lines indicate Mamu A*01 positive animals and solid lines indicate Mamu A*01 negative animals. (E) Median temporal levels of plasma viral RNA in animals that peaked at week 2 and > week 3. (F) Comparison of colorectal virus at 2 weeks post challenge by time of peak in plasma. Key to animal names is presented in Figure 3B. Boxes represent medians with 25^th and 75^th percentiles for the group. (G) Correlation between levels of viral RNA in the rectum at week 2 and plasma at weeks 2 and 24. Dryvax-naïve animals are shown in closed triangles (n= 8), Dryvax-immune in closed squares (n= 8) and controls in open triangles (n= 8). The sensitivity of viral load assay was 80 copies of RNA/ml, and animals with levels of virus below 80 were scored at 100. r_s, Spearman’s rank correlation.