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. 2012 Aug 6;3:151. doi: 10.3389/fphar.2012.00151

Figure 1.

Figure 1

Human AKR1C3 promoter fragment was subcloned into the pGL3 Basic vector and the CCAAT- and GC-elements were mutated and transiently transfected into HepG2 cells. The bar graph represents mean ± SD from at least three experiments. The luciferase expression was normalized to the transfection efficiency by β-galactosidase expression. When the CCAAT-box was mutated there was a borderline significant 50% increase in the relative luciferase activity. When the GC-box was mutated the luciferase activity significantly decreased indicating that the GC-box is required for the basal transcription of the AKR1C3 gene in HepG2 cells.