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. 2012 Aug 6;3:151. doi: 10.3389/fphar.2012.00151

Figure 2.

Figure 2

(A) Transcriptional activities of the AKR1C3 promoter construct transiently transfected into DSL2 cells. The DSL2 cells were cotransfected with Sp-proteins in increasing concentrations. The bar graph represents mean ± SD from at least three experiments. The luciferase expression was normalized to the transfection efficiency by β-galactosidase expression. Control background activity was increased twofold when Sp3 (0.5, 1, and 1.5 μg) were added to cells, whereas no significant affect was observed when increasing concentrations of Sp1 were added. (B) Human AKR1C3 promoter construct was transiently transfected into HepG2 cells cotransfected with Sp1/Sp3 proteins. The bar graph represents mean ± SD from at least three experiments. The luciferase expression was normalized to the transfection efficiency by β-galactosidase expression. When the Sp1 protein (1 μg) containing DSL cells were cotransfected with Sp3 (1.5 μg) the promoter activity increased 2.2-fold. When the Sp3 (1 μg) containing DSL cells were cotransfected with Sp1 (1.5 μg) no induction in promoter activity was observed.