Fig. 4.

Northern (RNA) blot analysis of fusin mRNA in different cell types. Polyadenylated RNA was prepared from the indicated cell types with the Micro-Fast Track Kit (Invitrogen). Samples (1 μg of RNA) were electrophoresed through a 1 % argarose-formaldehyde gel and transferred to a nitrocellulose membrane. A [α-³²P]guanosine triphosphate–labeled hybridization probe was prepared from the full-length fusin cDNA template by random primer synthesis. Hybridization was carried out with QuikHyb (Promega). The membrane was washed twice with 2% saline sodium citrate (SSC) and 0.1% SDS at room temperature for 30 min and once with 0.1 % SSC and 0.1% SDS at 65°C for 1 hour. Labeled bands were detected by autoradiography.