Table 1.

Primer and probe sequences tested.

Assay	Gene	Forward primer (5′ to 3′)	Reverse primer (5′ to 3′)	Probe (5′ to 3′)	Ref.
NL-N	N	CATATAAGCATGCTATATTAAAAGAGTCTC	CCTATTTCTGCAGCATATTTGTAATCAG	TGYAATGATGAGGGTGTCACTGCGGTTG	21
NL-N 2	N	CATAYAARCATGCTATATTAAAAGAGTCTC	CCTATYTCWGCAGCATATTTGTAATCAG	CAACHGCAGTRACACCYTCATCATTRCA	*
CDC	F	CAAGTGTGACATTGCTGAYCTRAA	ACTGCCGCACAACATTTAGRAA	TGGCYGTYAGCTTCAGTCAATTCAACAGA	19
CDC 2	F	CAARTGYGACATTGMTGAYCTRAA	AYTGCCGCACAACATTTAGRAA	CTTCTGTTGAATTGACTGAAGCTRACRGCCA	*
UR 2	N	CATGCTATATTAAAAGAGTCTCA	TCWGCAGCATATTTGTAATCAG	CAACHGCAGTRACACCYTCATCAATGCA	14 *
VU	N	ATGTCTCTTCAAGGGATTCACC	ATYTCTTGYTGCAATGATGARG	TCATAYAARCATGCTATATTAAAAGAGTCTCARTACACA	§

Primers were purchased from Invitrogen and were normal phase chromatography desalted. Probes were purchased from Operon and were reverse phase high performance liquid chromatography (HPLC) purified. Probes were 5′-labeled with 6-FAM and 3′-labeled with Black Hole Quencher-1 (BHQ-1).

^*= modified from published sequences as described in text; UR = University of Rochester; NL = Netherlands; VU = Vanderbilt University

^§= this study

H = A, C, or T; R = A or G; W = A or T; Y = C or T