Figure 2.

Lower expression of BLK mRNA in T cells and UCB B cells in rs922483 homozygous risk cells. BLK transcript was measured by qPCR in primary human B and T cell subsets and normalized to GAPDH. rs922438 homozygous A (risk allele) subjects are shown as open circles and homozygous G subjects are shown as filled circles. (A) Adult peripheral blood cell subsets: memory (CD19⁺CD3⁻CD27⁺) and mature/naïve (CD19⁺CD3⁻CD27⁻) B cells, CD4⁺, CD8⁺ and CD4⁻CD8⁻ γδ TCR⁺ T (CD3⁺CD19⁻) cells, (B) UCB cell subsets: naïve (CD19⁺CD3⁻CD27⁻CD10⁻CD38^int) and transitional (CD19⁺CD3⁻CD27⁻CD10⁺CD38^hi) B cells, CD4⁺ and CD8⁺ T cells. Statistically significant differences as determined by the Mann–Whitney non-parametric U-test to compare indicated groups are denoted as *P <0.05 and **P <0.01 and the mean is represented by a horizontal line.