Figure 4.

Blk protein levels are significantly reduced in umbilical cord naïve and transitional B cells from subjects homozygous for the risk allele. Blk was measured by intracellular staining and flow cytometry. The GMFI of cells labeled with anti-Blk was normalized to the GMFI of isotype labeled cells. Shown is the relative expression of Blk in cells gated using the following surface marker phenotypes: UCB naïve B cells (CD3⁻CD19⁺IgD⁺CD27⁻CD10⁻CD38^int), UCB transitional B cells (CD3⁻CD19⁺IgD⁺CD27⁻CD10⁺CD38^hi), UCB T cells (CD3⁺CD19⁻γδTCR⁻ and CD3⁺CD19⁻γδTCR⁺) and UCB CD3⁻CD19⁻ cells. Samples homozygous for rs922483 A (risk allele) are shown as open circles and homozygous G samples are shown as filled circles. Statistically significant differences as determined by the Mann–Whitney non-parametric U-test to compare indicated groups are denoted as *P <0.05 and **P <0.01 and the mean is represented by a horizontal line. The data are representative of three independent experiments.