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. 2012 Aug 6;2:89. doi: 10.3389/fonc.2012.00089

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Copyright © 2012 Chiang, Fu, Wang, Yu, Chen, Lin and Hong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Aggregated TAMs express higher level of Arg-1 than random TAMs. (A) The IHC staining for the expression of Arg-1 by random or aggregated CD68+ TAMs in control, irradiated (IR) or pre-irradiated (pre-IR) ALTS1C1 tumor Green: anti-Arg-1 antibody; red: anti-CD68 antibody. Merged images: the colocalization of Arg-1 and TAMs. Scale bar = 100 μm. (B) The percentage of Arg-1+ TAMs (left graph) and the mean intensity of Arg-1 staining (right graph) by random or aggregated CD68+ TAMs in control (rectangular dot region), irradiated (IR) (white bar) or pre-irradiated (pre-IR) (dark dot bar) ALTS1C1 tumors. The rectangular dot region represents the average value ± SD in control ALTS1C1 tumor for the purpose of clarity.