Figure 4.

(A) Tissue levels of the reduced folate after treatment with LV. Intratumoral reduced folate levels after LV administration were determined at 24 h after a single administration of vehicle alone (white column) or LV at 10 mg/kg (black column). Values are the mean ± SD of three mice. (B) Formation of the ternary complex after treatment with UFT and UFT/LV. Folate-depleted mice bearing Co-3 tumors were used for this experiment. Free TS protein and ternary complexes were detected using Western blotting at 24 h after a single administration of vehicle alone, or UFT (20 mg/kg) alone or in combination with LV (10 mg/kg).