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. 2012 May 22;20(8):1540–1549. doi: 10.1038/mt.2012.102

Figure 1.

Figure 1

Macromolecule transduction domain (MTD)- and protein transduction domain (PTD)-mediated protein delivery into cells and tissues. (a) Uptake of fluorescein isothiocyanate (FITC)-labeled proteins by RAW264.7 cells. Cells were exposed to the indicated proteins (10 µmol/l) for 1 hour, treated to remove cell-associated but noninternalized protein and analyzed by flow cytometry. The geometric means of FITC fluorescent peaks are plotted. (b) Uptake of FITC-labeled proteins by white blood cells. FACS profiles of WBC prepared from mice after intraperitoneal (i.p.) injection (300 µg/head) [FITC only (shaded); Hp18 (light green) and the indicated proteins (other colors)]. (c) Western blot analysis of proteins in serum, liver, and spleen. (d) Liver and tissue distribution of recombinant proteins. Tissue sections from mice were prepared 2 hours postinjection and immunostained with anti-p18INK4c antibody.