Figure 3.

Generation of recombinant adeno-associated virus (rAAV) serotype 1, 2, 8, 9- specific Th1-type immunoglobulin G (IgG) subtypes are strongly reduced in MyD88-deficient mice and perturbed in TLR9^−/− mice. Mice [wild type (wt), MyD88^−/−, or TLR9^−/− mice] were immunized intravenous (i.v.) with 10¹¹ vg of rAAV1-U7mEx23, rAAV2-U7mEx23, rAAV8-mSeAP or rAAV9-mSeAP (controls received PBS) and sera were harvested at day 35 for the measurement of respectively AAV1, AAV8, AAV9-specific IgG subtypes by ELISA. Curves represent the mean ± SEM of optical density obtained at the indicated dilutions. Statistical analyses were performed by comparing area under the curve values using Student's t-test. (a) Representation of four independent experiments (n = 13 mice per group). Top and bottom panels represent the comparison of wt (plain line) mice with respectively MyD88^−/− or TLR9^−/− mice (dotted lines). Responses were obtained in same sets of experiments but separated for clarity. (b) Grouped representation of data from three mice per group (wt = plain gray line; MyD88^−/− = black dotted line; TLR9^−/− = gray finely-dotted line). From top to bottom, respectively responses to rAAV2, rAAV8 and rAAV9.