Figure 7.

Free short interfering RNA (siRNA) diffuses into HeLa-Luc cells permeabilized with PVBLG_n-8. (a) In vitro transfection of HeLa-Luc cells with in situ formed complexes of PVBLG₁₀₀-8 and luciferase siGL3 at a variety of polymer:siRNA weight ratios and siRNA concentrations. (b) Fluorescence microscopy of HeLa-Luc incubated with complexes formed with TAMRA-labeled siRNA and PVBLG₁₀₀-8. Free polymer and free siRNA were added sequentially in the pane i. Free polymer was added, incubated and removed prior to addition of free siRNA in pane ii. Free siRNA was incubated without any poymer in pane iii. (c) In vitro transfection of HeLa-Luc cells with free luciferase siGL3 following incubation and removal of free PVBLG₁₀₀-8 at a variety of effective polymer:siRNA weight ratios and siRNA concentrations.