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. 2012 Jun 5;20(8):1582–1589. doi: 10.1038/mt.2012.33

Figure 1.

Figure 1

ApoB mRNA silencing by A-lip-chol-siApoB complexes. (a) In vivo apoB mRNA silencing activity of A-lip-chol-siApoB-1 and A-lip-chol-siApoB-2. For comparison, apoB mRNA silencing with chol-siRNA alone and chol-siApoB complexed with purified mouse HDL (mHDL) are shown. Results were shown relative to PBS control animals. N = 4 per group. Each value represents the group mean ± SD. *P < 0.05. (b) Binding of A-lip-chol-siApoB-1 and A-lip-chol-siApoB-2 to rat hepatocytes in the presence of a competitor (rat HDL, nonspecific IgG, or control PBS) using 32P- labeled chol-siRNAs at 4 °C. The assay was done in triplicate. Each value represents the group mean ± SD. (c) ApoB mRNA silencing by A-lip-chol-siApoB complexes in vitro. Mouse primary hepatocytes were incubated with 0.63 µmol/l of A-lip-chol-siApoB-1 or A-lip-chol-siApoB-2 for 6 hours at 37 °C and ApoB mRNA was quantified by qPCR. The assay was done in triplicate. Each value represents the group mean ± SD. apoB, apolipoprotein B; chol-siRNA, cholesterol-conjugated small interfering RNA; cpm, counts per minute; HDL, high-density lipoprotein; mRNA, messenger RNA; PBS, phosphate-buffered saline; qPCR, quantitative PCR.