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Expression and purification of rOPN-GST fusion protein. rOPN was produced as a fusion protein with GST in E. coli BL21. (Left panel) Purified rOPN-GST was separated by 12% SDS-PAGE and stained with Coomassie brillant blue. (Right panel) The separated fusion proteins were transferred onto nitrocellulose membranes and then incubated with an anti-OPN monoclonal antibody.
