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. Author manuscript; available in PMC: 2012 Aug 6.
Published in final edited form as: Mol Microbiol. 2011 Aug 4;81(5):1166–1177. doi: 10.1111/j.1365-2958.2011.07781.x

Fig. 2.

Fig. 2

Like its Hfx. volcanii counterpart, the Har. marismortui S-layer protein is glycosylated. The protein contents of Hfx. volcanii (left) and Har. marismortui (right) cells grown to identical OD550 values separated by 7.5% SDS-PAGE and either Coomassieor PAS-stained [left (CBB) and middle panels respectively]. In addition, the protein contents of Hfx. volcanii (left) and Har. marismortui (right) cells challenged with [2-3H]-mannose were separated by 7.5% SDS-PAGE and processed for fluorography (right panel). In each panel, the Hfx. volcanii and Har. marismortui S-layer glycoproteins are indicated by an arrow. Molecular mass markers are shown on the left of the figure.