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. Author manuscript; available in PMC: 2012 Aug 6.
Published in final edited form as: Mol Microbiol. 2011 Aug 4;81(5):1166–1177. doi: 10.1111/j.1365-2958.2011.07781.x

Fig. 6.

Fig. 6

No pentasaccharide-modified dolichol phosphate accumulates in Hfx. volcanii cells lacking AglB nor is detected using aminopropyl resin for liquid chromatography.

A. Tetrasaccharide-modified C55 and C60 dolichol phosphate are detected upon LC-ESI/MS analysis of the total lipid extract from Hfx. volcanii parent strain (upper panel) and ΔaglB cells (lower panel). In neither case is pentasaccharide-modified dolichol phosphate detected. The peaks shown correspond to doubly charged [M–2H]2− ions, with the positions of the different glycan-modified dolichol phosphates indicated.

B. Tetrasaccharide-modified C55 and C60 dolichol phosphate are detected upon LC-ESI/MS analysis of the total lipid extract from Hfx. volcanii parent strain (upper panel) and ΔaglB cells (middle panel) using aminopropyl resin for the liquid chromatography step. In neither case is pentasaccharide-modified dolichol phosphate detected. In contrast, pentasaccharide-modified C55 and C60 dolichol phosphates are detected upon similar analysis of the total lipid extract from Har. marismortui cells (lower panel). The peaks shown correspond to [M–H]− ions, with the positions of the different glycan-modified dolichol phosphates indicated.