Figure 3.
Binding, mineralization, and structural characterization of mineral products of the ADPs. (a) Binding constants, KD, of the ADPs determined by QCM (see Supplementary Information). (b) Calcium consumption rates of mineralization in the presence of ADP5 and ADP7 and rM180. (c) Calcium phosphate minerals formed in solution in the presence of ADPs. (d) The mineral product of ADP7 resembles those formed in the presence of rM180 in solution and these acicular crystallites are consistent with HAp morphology. (e) XRD patterns of the minerals formed by ADPs. Materials formed by ADP7 and amelogenin display the characteristic peaks belonging to the HAp crystal structure, while all minerals formed by other ADPs display weak diffraction peaks, consistent with amorphous, or only loosely crystalline outcomes shown in (c). ADP, amelogenin-derived peptide; HABP, hydroxyapatite-binding peptide; HAp, hydroxyapatite; rM180, recombinant mouse 180 amino acid long amelogenin; XRD, X-ray diffraction.