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. Author manuscript; available in PMC: 2012 Aug 6.
Published in final edited form as: Cancer Res. 2011 Jun 6;71(15):5336–5345. doi: 10.1158/0008-5472.CAN-10-2633

Figure 6. Sna1 induces N-Myc in neural cells.

Figure 6

A. Western blot analyses revealed increased expression of N-Myc in medulloblastoma cells and GCPs expressing Sna1. β-catenin is induced by Sna1 only in Daoy cells. CyclinD1 was unaffected by Sna1. B. RT-PCR shows increased expression of N-Myc in medulloblastoma cells expressing Sna1. C. A ChIP assay revealed that Sna1 binds to the N-Myc promoter between positions −1651 and −932. D. Daoy cells transfected with the wild-type N-Myc promoter (WT) driving luciferase expression show a 258% increase in promoter induction by Sna1 as compared to the control. Mutation of E-box sequences in the region of Sna1 binding prevents N-Myc promoter by Sna1. E. Daoy cells transfected with shRNA against Sna1 show a 75% reduction in N-Myc promoter activity compared to scrambled shRNA.