Figure 3.

HER2 downregulation by phenethyl isothiocyanate (PEITC) leads to mitochondrial death pathway. (A) Time-dependent effect of 10 μM PEITC in MDA-MB-231 cells. (B) PEITC treatment mediated cytochrome c release in the cytosol. Mitochondria-free cytosol was used to detect cytochrome c in control and treated cells. Actin was used as loading control. (C) Representative images of HER2 immunofluorescence in MDA-MB-231 in control and PEITC treated cells. Each experiment was repeated three times independently.