Figure 1.

Spore density-dependent AF productions inA. flavusin PMS media. (A, B), TLC analyses of AF productions by A. flavus A3.2890 cultured in GMS (A) or PMS (B) media for 3 days with initial spore densities of 10¹, 10², 10³, 10⁴, 10⁵ and 10⁶ spores/ml. Ten μl AF extracts were loaded in (A), and 50 μl in (B). St: AF standards. (C, D) HPLC analyses of AFs produced by A. flavus A3.2890 cultured in GMS (C) or PMS (D) media for 3 days, with the initial spore densities of 10¹, 10², 10³, 10⁴, 10⁵ and 10⁶ spores/ml. Note in GMS media both AFB1 and AFG1 were produced, while in PMS media mainly AFG1 was produced. (E) The time course of AFG1 productions in PMS media during 5-day cultures, with initial spore densities of 10⁶ (dotted line) or 10⁴ (solid line) spores/ml. All results were the mean ± SD of 3 measurements from mixed three independent samples.