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Additions of TCA cycle intermediates can not restore the AF biosynthesis in high initial spore density cultures. In A. flavus A3.2890 mycelia grown in PMS media initiated with 104 and 106 spores/ml, 0.5 mM or 5 mM TCA cycle intermediates, fumaric acid, malic acid and succinic acid, were added at the beginning of the culture. AFs were extracted from media and analyzed by TLC after 3-day cultivations.
