Table 1.
Metabolites differ between high and low spore density cultures
|
Groups |
Metabolites |
R. T. |
Relative contents |
Relative contents |
104/106 |
|---|---|---|---|---|---|
| (sec) | in 104spores/ml* | in 106spores/ml* | ratio | ||
|
TCA cycle intermediates |
Malic acid |
587.5 |
0.0187 ± 0.0001 |
0.0098 ± 0.0027 |
1.91 |
| Fumaric acid |
1349.0 |
0.0869 ± 0.0090 |
0.0509 ± 0.0043 |
1.71 |
|
| Succinic acid |
269.8 |
0.0103 ± 0.0020 |
0.0043 ± 0.0016 |
2.4 |
|
|
Amino acids |
Glycine |
417.0 |
0.0066 ± 0.0028 |
0.0034 ± 0.0003 |
1.94 |
| Phenylalanine |
713.7 |
0.1649 ± 0.0330 |
0.0084 ± 0.0007 |
19.63 |
|
| Proline |
410.0 |
0.0552 ± 0.0179 |
0.0099 ± 0.0009 |
5.58 |
|
| Valine |
331.5 |
0.0191 ± 0.00028 |
0.0088 ± 0.0006 |
2.17 |
|
| Tyrosine |
964.1 |
0.0430 ± 0.0090 |
0.0242 ± 0.0027 |
1.78 |
|
| Isoleucine |
404.0 |
0.0059 ± 0.0017 |
0.0042 ± 0.0003 |
1.4 |
|
|
Fatty acids |
Palmitic acid |
1054.5 |
0.3447 ± 0.037 |
0.2640 ± 0.011 |
1.31 |
| Stearic acid |
1212.4 |
0.2218 ± 0.027 |
0.1402 ± 0.0133 |
1.58 |
|
| Oleic acid |
1193.0 |
0.0833 ± 0.0024 |
0.0744 ± 0.0033 |
1.12 |
|
| Linoleic acid |
1188.8 |
0.8959 ± 0.0671 |
0.6315 ± 0.0554 |
1.42 |
|
|
Nucleotides |
Pyrimidine |
449.0 |
0.0154 ± 0.0044 |
0.0039 ± 0.0004 |
3.95 |
| Purine |
630.8 |
2.1901 ± 0.3141 |
1.3194 ± 0.0221 |
1.66 |
|
|
AA metabolism |
Pentanediamine |
881.1 |
0.1703 ± 0.0143 |
0.0162 ± 0.0011 |
10.51 |
| Amine |
369.0 |
0.0734 ± 0.0261 |
0.0328 ± 0.0036 |
2.24 |
|
|
Sugar metabolism |
Ribitol |
784.2 |
0.6276 ± 0.1768 |
2.6039 ± 0.1502 |
0.24 |
| |
Glucopyranoside |
1561.8 |
0.1130 ± 0.0198 |
0.3344 ± 0.0354 |
0.34 |
| |
Gluconolactone-6-P |
821.6 |
0.5679 ± 0.0839 |
0.7094 ± 0.0181 |
0.80 |
| |
Glycerol |
986.0 |
0.0073 ± 0.0015 |
0.0103 ± 0.0009 |
0.71 |
| |
Butanediamine |
801.1 |
0.0656 ± 0.0086 |
0.1224 ± 0.0051 |
0.54 |
| |
Ehylamine |
563.0 |
0.2082 ± 0.0320 |
0.2436 ± 0.0013 |
0.85 |
| Galactose | 948.1 | 1.6122 ± 0.4037 | 1.9547 ± 0.3306 | 0.82 |
*Values represent metabolites abundances (mean ± SD) of 3 replicates from mixed 3 independent samples, as measured by GC-Tof-MS in 3-day mycelia (normalized by C17:0 fatty acid). R.T.: retention time; TCA: tricarboxylic acid; AA: amino acid.