Figure 1.

Chemical structures of adenosine-5′-triphosphate (ATP), Fasudil and H-89 in similar orientations with respect to the ATP pocket (hinge region at the left). The isoquinoline portion of H-89 is highlighted in blue, the sulfonamide portion in green and the bromobenzene moiety in red. The numbering of the H-89 molecule refers to C3 and N4, consistent with the designation of the atoms in the structure 3vqh. ‘PKA’ refers to cAMP-dependent protein kinase catalytic subunit α isoform 1 and ‘ROCK2’ to Rho kinase α. Affinity values were taken from the literature: *, Rajagopalan et al. (2010 ▶); †, Gassel et al. (2003 ▶); ‡, Yano et al. (2008 ▶); §, Engh et al. (1996 ▶).