Figure 5. Life cycle stage triage.

A. Performance (EC₅₀) of control entry (EI), genome replication (BMS-339) and late-stage (LY411575) inhibitors in single- vs. multi-cycle replication assay formats. B. Screen hits (Inhs 1–13) that demonstrated similar potency in single- and multi-cycle replication formats (single- & multi-cycle inhibitors). C. Screen hits (Inhs 14–17) that demonstrated greater potency in multi-cycle replication assay format (multi-cycle specific inhibitors). D. Performance of control entry (EI) and genome replication (BMS-339) inhibitors following infection with gt 1a/2a-Rluc virus or direct transfection of the corresponding virus genomic RNA. E. Screen hits (Inhs 1–5) that demonstrated reduced potency following direct transfection of virus genomic RNA (early inhibitors). F. Screen hits (Inhs 6–13) that demonstrated similar potency in infection and transfection assays (genome replication inhibitors).