Table 2. Primary Screen Results.
| Screening Tier | Criteria | Pass | Fail |
| Primary Screen & Re-Testsa | ≥70% inhibition/≤30% cytotoxicity at 6 µM compound. Removed known HCV actives. | 9,025 | 1,166,479 |
| Potency Filter | ≥30% inhibition at 0.6 µM compound. | 632 | 8,865 |
| Dose-Response Curves | EC50≤1 µMb | 386 | 246 |
| Therapeutic Index ≥10c | CC50/EC50 ≥10 | 307 | 79 |
| Renilla luciferase counterscreen | No activity against Renilla luciferase VSV pseudo-typed reporter retrovirus. | 232 | 75 |
| Chemical analysis | Compounds grouped by similarity. Representative for each class advanced. | 17 | - |
| Hit Confirmation | Similar EC50 using Renilla luciferase and Cellomics assay read-outs. | 17 | 0 |
a
Single-point (6 µM); Re-tests in triplicate.
b
Mean EC50 achieved from 10-point dose-response curve; in triplicate.
c
CellTiter-Glo cytotoxicity assay.