Table 1. Proportion of human immune cells in hNOK/B51Tg and hNOK mice.
| % nucleated cells | ||||||||
| Donor | hCD45 gated | hCD45/CD3 gated | ||||||
| cord blood | No. | mouse | sex | hCD45+ | CD3+ | CD19+ | CD4+ | CD8+ |
| 1 | 1 | NOK | F | 1.68 | 88.1 | 7.05 | 59.4 | 40.3 |
| 2 | NOK | F | 9.34 | 84.7 | 8.28 | 54.7 | 44.3 | |
| 3 | NOK | M | 5.13 | 98.6 | 0.36 | 61.6 | 37.6 | |
| 4 | NOK | M | 1.93 | 93.5 | 2.01 | 43.4 | 56.3 | |
| 2 | 1 | NOK/B51 | F | 5.69 | 22.6 | 70.3 | 36.0 | 58.6 |
| 2 | NOK/B51 | F | 2.46 | 61.6 | 34.5 | 31.6 | 66.4 | |
| 3 | NOK/B51 | F | 7.92 | 10.2 | 83.0 | 20.6 | 74 | |
| 4 | NOK/B51 | F | 7.66 | 21.3 | 74.6 | 20.3 | 69.3 | |
| 5 | NOK/B51 | M | 4.2 | 27.8 | 63.6 | 33.9 | 55.2 | |
| 3 | 1 | NOK/B51 | F | 2.76 | 27.1 | 47.7 | 75.9 | 19.1 |
| 2 | NOK | M | 1.06 | 32.6 | 46.1 | 71.9 | 19.0 | |
| 3 | NOK/B51 | M | 14.8 | 74.9 | 19.2 | 49.0 | 42.0 | |
| 4 | 1 | NOK/B51 | M | 2.15 | 70.8 | 20.6 | 52.4 | 38.9 |
| 5 | 1 | NOK/B51 | F | 3.3 | 72.2 | 16.1 | 34.7 | 51.4 |
| 6 | 1 | NOK/B51 | M | 9.77 | 98.3 | 0.58 | 31.4 | 53.8 |
| 7 | 1 | NOK/B51 | F | 8.76 | 6.87 | 81.5 | 67.1 | 31.5 |
| 2 | NOK | F | 3.21 | 33.6 | 53.4 | 44.4 | 54.8 | |
| 3 | NOK/B51 | M | 1.36 | 44.2 | 46.2 | 36.4 | 61.4 | |
| 4 | NOK/B51 | M | 7.13 | 21.5 | 65.7 | 59.2 | 39.8 | |
| 5 | NOK | M | 3.72 | 61.7 | 28.7 | 47.2 | 52.2 | |
| 8 | 1 | NOK | M | 19.5 | 66.7 | 25.3 | 59.2 | 35.8 |
| 9 | 1 | NOK/B51 | F | 17.7 | 75.2 | 20.9 | 46.7 | 53.4 |
| 10 | 1 | NOK | M | 3.0 | 29.4 | 46.5 | 24.0 | 76.0 |
| 11 | 1 | NOK/B51 | M | 10.6 | 48.3 | 39.5 | 50.7 | 45.3 |
| 12 | 1 | NOK/B51 | F | 29.3 | 71.1 | 25.7 | 77.4 | 17.7 |
| 2 | NOK/B51 | M | 5.83 | 41.2 | 49.3 | 52.8 | 44.0 | |
| 13 | 1 | NOK/B51 | M | 6.77 | 28.4 | 58.1 | 46.0 | 54.0 |
| 2 | NOK | M | 2.98 | 67.5 | 22.7 | 35.6 | 60.6 | |
| 14 | 1 | NOK | M | 44.0 | 60.8 | 36.9 | 75.2 | 17.7 |
| 15 | 1 | NOK | F | 6.52 | 27.1 | 67.8 | 47.5 | 43.8 |
| 16 | 1 | NOK/B51 | F | 4.03 | 19.5 | 73.2 | 37.6 | 61.7 |
| 2 | NOK | M | 8.36 | 13.5 | 76.7 | 24.1 | 73.0 | |
| 17 | 1 | NOK | F | 29.5 | 51.7 | 45.2 | 44.1 | 53.1 |
| 2 | NOK | F | 29.6 | 62.1 | 33.9 | 49.3 | 49.3 | |
| 3 | NOK | M | 11.4 | 53.6 | 43.2 | 27.1 | 68.5 | |
For generation of hNOK/B51Tg and hNOK mice, human CD34+ cells derived from 17 cord blood samples were transplanted into newborn NOK/B51Tg and NOK mice, respectively. PBMCs from hNOK/B51Tg (n = 19) and hNOK (n = 16) mice were analyzed for the proportion of human immune cells at 10 weeks after the transplantation of human CD34+ HSCs.