. 2012 Aug 6;7(8):e42668. doi: 10.1371/journal.pone.0042668

Table 3. Comparison of group least squares means (± SEM) of hematopoietic cellularity, adiposity, and bone volume in intact (I) and ovariectomized (OVX) mice without radiation (−R) and with radiation (+R) 30 days after radiation treatment.

Treatment group	% Hematopoietic cellularity (SEM)	% Adipose (SEM)	%BV/TV (SEM)
I−R	96.2 (3.0)^a	1.11 (0.72)^a	5.61 (0.86)
I+R	72.0 (3.0)^a,c	3.55 (0.72)^b	2.63 (0.86)
OVX−R	93.0 (3.4)^b	4.97 (0.81)^a,c	4.53 (0.96)
OVX+R	56.0 (3.0)^b,c	12.14 (0.72)^b,c	3.66 (0.86)

Within columns, identical letters indicate relevant statistically significant differences between groups at p≤0.001). Radiation significantly reduced hematopoietic cellularity in intact mice, but the effect was more severe in OVX (interaction F = 4.79, p = 0.0439). OVX alone had no effect on hematopoietic activity in the absence of radiation. Radiation significantly increased marrow adipose only in OVX (interaction F = 10.14, p = 0.0062), and OVX increased marrow fat without and with radiation treatment. Radiation significantly reduced BV/TV% (p = 0.0467), but OVX had no additive effect. All statistical analyses were based on two-way ANOVA.