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. 2012 Aug 6;7(8):e42536. doi: 10.1371/journal.pone.0042536

Figure 4. TBCA16 protein is absent in early stages of mouse spermatogenesis.

Figure 4

Protein extracts from mouse testis in early stages of spermatogenesis (14 post-natal days – PND14) and recombinant TBCA13 and TBCA16 proteins produced and purified from bacteria were analyzed by 16.5% (w/v) Tricine–SDS–PAGE followed by western blot with a specific polyclonal antibody directed to TBCA. Note that this antibody recognizes both recombinant proteins. Under these conditions, TBCA13 and TBCA16 proteins have distinct motilities. The approximate molecular mass of the proteins is indicated at the left side of the panels. The regions around the 14 kDa marked by dashed squares were excised and proteins present in these regions were analyzed by electrospray mass spectrometry (MS/MS). The analyses lead to the identification of the TBCA13 specific peptide R.LEAAYTDLQQILESEK.D (the specific aminoacid is underlined). No TBCA16 specific peptides were identified (see table).