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. 2012 Aug 6;7(8):e42536. doi: 10.1371/journal.pone.0042536

Figure 6. Tbca16 knockdown increases the steady-state levels of Tbca13 RNA in GC-2spd(ts)-spermatocyte mouse cell line.

Figure 6

After 48 h of transfection, total RNA was extracted from GC-2spd(ts) cells expressing non-target shRNA, TbcashRNA(knockdownTbca13+Tbca16) or Tbca16shRNA (to knockdown exclusively the Tbca16 RNA). Semi-quantitative RT-PCR analysis showed a decrease in the steady-state levels of Tbca13 and Tbca16 mRNAs in Tbca shRNA expressing cells in comparison to those in cells expressing non-target shRNA. However, in cells expressing Tbca16 shRNA the steady-state levels of Tbca16 mRNA decrease whereas those of Tbca13 mRNA increase. Normalized cDNA is expressed as a percentage of maximal value (100%). *p<0,05 compared with control. The graphic bars are the mean±s.d. (error bars) of three independent assays. Values were standardized with those of Hprt cDNA. Statistical significance was calculated using a t-test.