Table 3. List of primers used to amplify long and short fragments of C. fletcheri mitochondrial genome.
Fragment | Primer name | Direction | Primer sequence (5′ to 3′) | Position* |
SF1 | Der-CO3F1 | F | CWATAATYCAATGATGACG | 7778 ∼ 7796 |
Der-CO3R1 | R | GTCCATGRAATCCTGTTGCTA | 8216 ∼ 8236 | |
SF2 | Der-srRNAF1 | F | CTACTWTGTACGACTTATCTC | 17892 ∼ 17913 |
Der-srRNAR1 | R | TAAACTAGGATTAGATACCCC | 18297 ∼ 18317 | |
LF1 | CF-CO3F2 | F | GCATCAGGGGTTACAGTTACTTG | 8035 ∼ 8057 |
CF-srRNAR1 | R | GAAAATGAATTCTCGACGAATAC | 18009 ∼ 18031 | |
LF2 | CF-srRNAF3 | F | AATCTTAAGACGACGGTATACAAGC | 18134 ∼ 18158 |
CF-CO3R2 | R | CACAAACAGGAGAAAGACTTC | 7931 ∼ 7951 |
Position of primer sequences corresponds to C. fletcheri mitogenome; F, forward; and R, reverse.