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. 2012 Aug 6;7(8):e42283. doi: 10.1371/journal.pone.0042283

Figure 3. Identification of putative natural self antigen.

Figure 3

A) Bidimensional electrophoresis gel stained with colloidal Coomassie Brilliant Blue. Sixty five µg of purified carotid atherosclerotic plaque proteins were loaded on strip pH 3-10NL, 7 cm. The 2nd dimension was carried out using 12.5% SDS-PAGE. B) 2D electrophoresis Western Blotting. Similarly, 70 µg of proteins were loaded on strip pH 3-10NL, 7 cm. The 2nd dimension was carried out using 12.5% SDS-PAGE. After transfer, proteins were probed with Fab7816-FLAG (10 µg/mL). Protein spots of interest (red box) were excised from the gel, digested with trypsin and analysed by MALDI-ToF mass spectrometry. In both spots human TAGLN was identified with almost complete sequence coverage.