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. 2012 Aug 6;7(8):e42283. doi: 10.1371/journal.pone.0042283

Figure 8. Western Blotting of bacterial lysates and on bacterial OMPs with Fab 7816.

Figure 8

A) Western blotting of bacterial lysates with Fab7816. 1 µg of each bacterial lysate was loaded in each lane. Fab 7816 clearly reacted with Proteus mirabilis and Klebsiella pneumoniae lysates. B) Western blotting on induced/non induced BL21(DE3) bacterial cells transformed with pET28b vector expressing OmpK36. Two pET vectors were constructed: the NcoI/XhoI wild type OmpK36 vector or the HIS-tagged Ompk36 BamHI/XhoI vector. In both cases Fab 7816 recognized only IPTG induced bacteria.