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. 2012 Aug 6;7(8):e42527. doi: 10.1371/journal.pone.0042527

Figure 1. TGFβ3 treatment increases stiffness of AV cushions through Alk5 mediated pathway.

Figure 1

A) Representative pipette test data for TGFβ3 (1 ng/ml, TGFβ3+) and control media (TGFβ3−) treated cushions, n = 4. Strain energy density was calculated from the shaded regions beneath the ΔP vs λ curves. Inset: image of aspirated HH25 AV cushion after 24 hours of culture. The pipette radius, rp, and the aspirated length, L are indicated. Scale bar = 70 µm. B) AV cushion strain energy density increased with TGFβ3 treatment, but was blocked by Alk5 inhibition (SB431542, 2.6 µM). mean ± SEM, n≥7, *p<0.0001, 2-way ANOVA.