Skip to main content
. 2012 Aug 6;7(8):e42527. doi: 10.1371/journal.pone.0042527

Figure 3. Remodeling behavior of TGFβ3 treated cushions is a balance of contractile differentiation, proliferation, and matrix synthesis.

Figure 3

A) 24 hour TGFβ3 treated cushions upregulate contractile (αSMA, RhoA), proliferation (cyclin b), and extracellular matrix protein (col1α2) encoding genes. TGFβ3 administration also significantly stimulated its own production. mean ± SEM, n = 3–4 pooled samples of 8–10 cushions, *p<0.05, t-test. B) BrdU incorporation data (red) of TGFβ3 treated cushions normalized to DRAQ5 cell nuclei counter stain (blue). BrdU was administered 6 hours prior to completion of 24 hour treatment. Representative confocal images are shown above each bar, with a global view of cushion contained in the inset. mean ± SEM, n = 12, *p<0.0001, t-test.