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. 2012 Aug 6;7(8):e42527. doi: 10.1371/journal.pone.0042527

Figure 5. 5-HT treatment modulates TGFβ3 mediated gene expression.

Figure 5

A) TGFβ3 mRNA transcripts increase with physiological 5-HT (470 nM, 5-HT), but decrease at high dose (47 µM, 5-HT+). αSMA, RhoA, and col1α2, were not affected by physiological 5-HT dose, but were significantly downregulated with high 5-HT treatment. B) High 5-HT treatment mitigates exogenous TGFβ3 induced contractile gene expression, while TGFβ3 induced proliferation was independent of 5-HT dose. mean ± SEM, n = 3–5 pooled samples of 8–10 cushions, *p<0.05 via ANOVA comparisons with controls.