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A) The strain energy density (Pa) of HH25 cushions increased 1.4 fold with systemic 5-HT treatment in ovo, mean ± SEM, n = 8–10 cushion, *p<0.05, t-test. B) Gene expression levels of HH25 AV cushions isolated from embryos treated with 5-HT at HH17 (48 hours). mean ± SEM, n = 6–10 samples, each of 8–10 pooled HH25 cushions, *p<0.05, t-test.
