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A) Representative images of pSmad2/3 staining. Arrows indicate pSmad2/3 positive cells. Cell nuclei – blue, pSmad2/3 – red. B) Embryos treated with systemic 5-HT at HH17 have increased pSmad2/3 expression at HH25 indicating elevated TGFβ signaling. n = 6, mean ± SEM *p<0.01, t-test.
