Figure 4. Protein expression of the androgen receptor (AR), human epidermal growth factor receptor 2 (Her-2/neu) and ratios of pAKT/AKT and pERK/ERK detected using A) Western blotting analysis in androgen-independent (AI) variants.

Actin was used as a control for loading protein. B) Histograms representing the quantification of AR, Her-2/neu and ratios of pAKT/AKT and pERK/ERK normalized to actin and mean of PAC120 coming from various passages (expressed arbitrary units). AR (black bars) was expressed in PAC120 and all AI variants and Her-2/neu (dotted grey bars) was overexpressed in all AI variants. Either p-AKT/AKT (grey bars with full line) or pERK/ERK (white bars) were activated in AI variants. Abbreviations used to identify variants are explained in the legend of Figure 2.