Table 2.

Description of genetically modified mouse models with subsequent variation in gene expression and studies involving those models

	sHSPs coregulation*			Other genes	Experimental conditions
	Up ↑	Down ↓	No change
HSF1
-KO – Hsf1tm1Ijb (1)	ND	-HSPB1 (3.3 x)	ND	HSPA1 (↓1.5 x)	-basal conditions
		-HSPB5 (1.6 x)
-KO – Hsf1tm1Anak (2)	ND	ND	ND	HSPA1 (↓)	-basal conditions, TAC
-KO – Hsf1tm1Miv (3)	ND	-HSPB1 (1.1 x)	ND		-basal conditions
-TG (ΔHSF1)(4)	HSPB1 (2.2 x)	ND	ND	HSPA1 (↑1.85 x)	-basal conditions. I/R, cardiac hypertrophy (TAC, exercise)
HSPB1
KO – HspB1tm1Msk (5)	-	-	-HspB2,3,5,6,7,8 (mRNA) -HSPB5	HSPA1 (no change)	-basal conditions
-TG(pCAGGS-hHSPB1) (6)
TG WT (↑100 x)	-mHSPB1 (0.3 x)	ND	-HSPB5	ND	-basal conditions, I/R (in vitro)
TG mut (P sites)(↑76 x)	-mHSPB1 (0.13 x)	ND
-TG(αMHC-hHSPB1) (7)
TG 85 (↑5.87 x)	ND	HSPB1 (3 x)	HSPB5	ND	-basal conditions, DOXO treatment
TG 21 (↑10.6 x)
TG 10 (↑11.7 x)	ND	ND	ND	ND
HSPB5
-TG(pCAGGS-rHSPB5) (8)					-basal conditions, I/R
TG (↑6.9 x)	ND	ND	HSPB1	ND
-TG(αMHC-mHSPB5) (9)					-basal conditions, R120G control lines, TAC
TG 11 (↑4.3 x)	ND	ND	ND	ND
-TG(αMHC-hHSPB5) (10)
TG (↑1.56 x)	-	HSPB1 (0.8 x)	ND	HSPA1(0.4x)	-basal conditions R120G control lines
- TG (αMHC-mHSPB5R120G) (9)(15)
TG (↑ 2.5 x)	HSPB1(2.57x) HSPB8 (2.4x)	-	-	HSPA1(↑1.35x)	-basal conditions
-TG(αMHC-hHSPB5 R120G) (10)
TG (↑ 12.5 x) (6month old)	HSPB1 (3.65x)	-	-	HSPA1(↑1.33x)	-basal conditions
-KI HspB5R120G(11)
-DKO with HspB2 (12)	HSPB1 (1.1 x)	ND	ND	ND	-basal conditions
HSPB2 and 5 (0 x)					-basal conditions, I/R, TAC
HSPB6
TG(αMHC-hHSPB6) (13)
TG Hsp20 WT (↑10 x)	ND	ND	HSPB1, HSPB5	ND	-basal conditions, I/R, DOXO treatment
TG Hsp20S16A(↑7 x)	ND	ND	HSPB1, HSPB5	ND
HSPB8
- TG (αMHC-hHSPB8) (14)
TG low (↑2 x), medium	HSPB1 (4.5 x)	ND	ND	HSPA1 (↑11 x)	-basal conditions, I/R
(↑4 x) high (↑7 x)
-TG (αMHCtetO-HSPB8)(15)
TG tTAxTG HSPB8 (↑2 x)	-	-	HSPB1, HSPB5	-	-basal conditions, R120G triple TG
-KO - HspB8(16)	ND	ND	ND	HSPA1 (↑3 x)	-basal conditions, TAC

References:

¹Yan et al (2002).

²Sakamoto et al. (2006).

³Zhang et al. (2002).

⁴Zou et al. (2003).

⁵Huang et al. (2007).

⁶Hollander et al. (2004).

⁷Liu et al. (2007) and Zhang et al. (2010).

⁸Ray et al., 2001.

⁹Wang et al. (2001) and Kumaparelli et al. (2008 and Kumaparelli et al. (2010).

¹⁰Rajasekaran et al. (2007).

¹¹Andley et al. (2011).

¹²Benjamin et al., 2007 and Kumaparelli et al. (2008 and Kumaparelli et al. (2010).

¹³Fan et al. (2005) and Qian et al. (2009).

¹⁴Depre et al. (2002).

¹⁵Sanbe et al. (2009).

¹⁶Qiu et al. (2011). No animal models have been published for HspB2, HspB3, HspB7.

^*Coregulation of sHSP level was measured under basal-normal conditions in the heart.

DOXO: exposure to doxorubicin. I/R: ischemia/reperfusion. Mut: mutant. ND: not determined. P sites: phosphorylation sites. TAC: transaortic constriction.