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Pro-antibody binding affinity is enhanced by proteolysis. (A) Binding activity of the anti-VCAM-1 pro-antibody before (○) and after (●) proteolysis, as measured by ELISA. Association and dissociation of (B) the anti-VCAM-1 antibody and (C) pro-antibody before (--) and after (-) proteolysis as measured by SPR.
