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Aortic plaque homogenates proteolytically activate pro-antibodies. (A) Anti-VCAM-1 Antibody and, (B) pro-antibody binding activity after incubation in assay buffer or with tissue homogenates from ApoE(−/−) or ApoE(+/+) mice, as measured by ELISA. Error bars represent the standard error of the mean of triplicate measurements. Significance was confirmed using both students T-test (shown), and one-way ANOVA analysis with Bonferroni’s multiple comparison test.
