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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Bone. 2012 May 19;51(3):535–545. doi: 10.1016/j.bone.2012.05.008

Figure 6. Analysis of the mineralized tissue and associations between vascular and osseous tissues.

Figure 6

(A) Volume of mineralized tissue in the total VOI (left graph) and volume fraction of mineralized tissue in the intraosteal VOI (right graph) for each time-point (postoperative day is indicated on the abcissa). Column heights and error bars indicate group means and standard deviations, respectively. *: p<0.05. (B) Representative histological progression of tissue development over the time-course of DO. Images are composites made from a montage of 100x micrographs taken in the mid-diaphyseal region. Thin arrows indicate the length and width of the gap region, inclusive of the robust periosteal response. Images are oriented so that the long axis of the bone is displayed approximately left to right in a proximal to distal manner. White scale bar represents 400 microns. (C) Higher-magnification (400x) micrographs of the intraosteal regions (top row) and muscular regions (bottom row) boxed in (B) show specific details to highlight aspects of vessel formation in the gap during active distraction and in the surrounding muscle at other time points. The region of muscle shown for POD 31 is out of the corresponding field of view shown in (B). Arrows denote specific vessel structures. White scale bar represents 100 microns.