Skip to main content
. Author manuscript; available in PMC: 2013 Sep 7.
Published in final edited form as: J Mol Biol. 2012 May 23;422(1):58–74. doi: 10.1016/j.jmb.2012.05.015

Figure 2.

Figure 2

GST-pulldown and Western blot analysis for WWOX-WBP2 interaction in mammalian cells. (a) HEK293 cells were transiently transfected with WBP2 and either wild type GST-tagged WWOX (WT) or GST-tagged WWOX containing the W44F/P47A double-mutation (FA). After harvesting for 24–30h, SDS-PAGE analysis was conducted on either whole cell lysate (Input) or after GST-pulldown and the resolved bands were detected by immunoblotting with anti-WBP2 (α-WBP2) and anti-GST (α-GST) antibodies. (b) HEK293 cells were transiently transfected with WBP2 and either GST-tagged WW1 domain (WW1) or GST-tagged WW2 domain (WW2) of WWOX. After harvesting for 24–30h, SDS-PAGE analysis was conducted on either whole cell lysate (Input) or after GST-pulldown and the resolved bands were detected by immunoblotting with anti-WBP2 (α-WBP2) and anti-GST (α-GST) antibodies.