Table 1.
Sequence | Kd/μM | ΔH/kcal.mol−1 | TΔS/kcal.mol−1 | ΔG/kcal.mol−1 | |
---|---|---|---|---|---|
WBP1_PY1 | NBD | NBD | NBD | NBD | |
WBP1_PY2 | 366 ± 23 | −16.14 ± 0.29 | −11.44 ± 0.25 | −4.69 ± 0.04 | |
WBP2_PY1 | NBD | NBD | NBD | NBD | |
WBP2_PY2 | NBD | NBD | NBD | NBD | |
WBP2_PY3 | 122 ± 1 | −12.61 ± 0.11 | −7.27 ± 0.12 | −5.34 ± 0.01 |
The PPXY motif is colored blue for clarity. Binding stoichiometries generally agreed to within ±10%. Errors were calculated from at least three independent measurements. All errors are given to one standard deviation. NBD indicates no binding determined for the particular peptides either due to lack of any observable heat change or due to poor accuracy of ITC analysis involving such weak interactions (Kd > 1 mM).