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. Author manuscript; available in PMC: 2013 Sep 7.
Published in final edited form as: J Mol Biol. 2012 May 23;422(1):58–74. doi: 10.1016/j.jmb.2012.05.015

Table 4.

Thermodynamic parameters obtained from ITC measurements for the binding of engineered WW2_RW domain of WWOX to various PPXY-peptides derived from WBP1 and WBP2

Sequence Kd/μM ΔH/kcal.mol−1 TΔS/kcal.mol−1 ΔG/kcal.mol−1
WBP1_PY1 graphic file with name nihms377220t17.jpg 80 ± 2 −5.59 ± 0.06 +0.01 ± 0.04 −5.60 ± 0.02
WBP1_PY2 graphic file with name nihms377220t18.jpg 123 ± 1 −12.11 ± 0.20 −6.77 ± 0.19 −5.34 ± 0.01
WBP2_PY1 graphic file with name nihms377220t19.jpg NBD NBD NBD NBD
WBP2_PY2 graphic file with name nihms377220t20.jpg 69 ± 1 −8.44 ± 0.06 −2.75 ± 0.06 −5.69 ± 0.01
WBP2_PY3 graphic file with name nihms377220t21.jpg 91 ± 2 −5.20 ± 0.04 −0.32 ± 0.03 −5.52 ± 0.01

Note that WW2_RW is a double-mutant of WW2 domain containing the E66R and Y85W substitutions. Binding stoichiometries generally agreed to within ±10%. Errors were calculated from at least three independent measurements. All errors are given to one standard deviation. NBD indicates no binding determined for the particular peptides either due to lack of any observable heat change or due to poor accuracy of ITC analysis involving such weak interactions (Kd > 1 mM).