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. Author manuscript; available in PMC: 2013 Sep 7.
Published in final edited form as: J Mol Biol. 2012 May 17;422(1):145–155. doi: 10.1016/j.jmb.2012.05.011

TABLE 4.

Antigenic distance changes between mutant influenza viruses and their respective parent influenza viruses.

Position a
Mutant b Antigenic Distance
Change c
Position a
Mutant b Antigenic
Distance
Change c
H5 H3 H1 H5 H3 H1
45 54(C) 48 04-VNM-D45N 1.5866 141 145(A) 145(Ca2) 04-VNM-S141F 2.4349
07-HKG-N45D 0.3897 04-VNM-S141Y 2.8910
83 91(E) 87 04-VNM-A83I 1.0408 162 166 166(Sa) 07-HKG-K162R 0.3901
05-MNG-I83A 1.5136 227 231 231 05-MNG-E227D 1.3612
94 101 98 04-VNM-D94N 0.0176 252 256 256 05-MNG-N252Y 1.8631
05-MNG-N94D 1.3984 263 266 267 05-MNG-T263A 1.6685
124 129(RBS) 129(Sa) 04-VNM-S124D 0.2685 282 285 286 04-VNM-M282I 1.0400
129 133(A) 133 04-VNM-L129S 2.6660 05-MNG-I282M 1.7717
140 144(A) 144 04-VNM-K140N 2.5963
a

The positions are numbered based on the amino-acid position in the HA of H5N1 HPAIV and corresponding positions in HA of H3N2 influenza A virus, respectively;

b

The 6+2 mutants generated by using site-directed mutagenesis and reverse genetics were named according to HA/NA donor-mutation, 04-VNM denotes A/Vietnam/1203/04(H5N1), 05-MNG A/whooper swan/Mongolia/244/05(H5N1), and 07-HKG A/common magpie/Hong Kong/5052/07(H5N1);

c

These antigenic distances were measured by using antigenic cartography 34; 35 (see FIG. 2). One grid unit corresponds to a 2-fold change in HI titer.