TABLE 4.
Antigenic distance changes between mutant influenza viruses and their respective parent influenza viruses.
| Position a |
Mutant b | Antigenic Distance Change c |
Position a |
Mutant b | Antigenic Distance Change c |
||||
|---|---|---|---|---|---|---|---|---|---|
| H5 | H3 | H1 | H5 | H3 | H1 | ||||
| 45 | 54(C) | 48 | 04-VNM-D45N | 1.5866 | 141 | 145(A) | 145(Ca2) | 04-VNM-S141F | 2.4349 |
| 07-HKG-N45D | 0.3897 | 04-VNM-S141Y | 2.8910 | ||||||
| 83 | 91(E) | 87 | 04-VNM-A83I | 1.0408 | 162 | 166 | 166(Sa) | 07-HKG-K162R | 0.3901 |
| 05-MNG-I83A | 1.5136 | 227 | 231 | 231 | 05-MNG-E227D | 1.3612 | |||
| 94 | 101 | 98 | 04-VNM-D94N | 0.0176 | 252 | 256 | 256 | 05-MNG-N252Y | 1.8631 |
| 05-MNG-N94D | 1.3984 | 263 | 266 | 267 | 05-MNG-T263A | 1.6685 | |||
| 124 | 129(RBS) | 129(Sa) | 04-VNM-S124D | 0.2685 | 282 | 285 | 286 | 04-VNM-M282I | 1.0400 |
| 129 | 133(A) | 133 | 04-VNM-L129S | 2.6660 | 05-MNG-I282M | 1.7717 | |||
| 140 | 144(A) | 144 | 04-VNM-K140N | 2.5963 | |||||
a
The positions are numbered based on the amino-acid position in the HA of H5N1 HPAIV and corresponding positions in HA of H3N2 influenza A virus, respectively;
b
The 6+2 mutants generated by using site-directed mutagenesis and reverse genetics were named according to HA/NA donor-mutation, 04-VNM denotes A/Vietnam/1203/04(H5N1), 05-MNG A/whooper swan/Mongolia/244/05(H5N1), and 07-HKG A/common magpie/Hong Kong/5052/07(H5N1);