Table I. List of differentially expressed proteins between LS+K and LS−K involved in immune response.
(* = quantified in two replicates)
| Uniprot ID | Gene Name | Protein Name | Biological Process/Molecular Function | Fold change LS+K/LS−K | Adjusted p value | # of peptides |
|---|---|---|---|---|---|---|
| Extracellular matrix | ||||||
| P29699 | Ahsg* | Alpha-2-HS-glycoprotein | Acute phase response; peptidase inhibitor activity | 5.42 | – | 2 |
| O35744 | Chi3l3 | Chitinase-3-like protein 3 | Bacterial carbohydrate sensing; chemotaxis | 4.85 | 0.00 | 7 |
| P11087 | Col1a1 | Collagen alpha-1(I) chain | Structural constituent; cell-substrate adhesion | 6.99 | 0.00 | 5 |
| Q01149 | Col1a2 | Collagen alpha-2(I) chain | Structural constituent; cell-substrate adhesion | 6.51 | 0.02 | 6 |
| P28481 | Col2a1 | Collagen alpha-1(II) chain | Structural constituent; cell-substrate adhesion | 5.63 | 0.04 | 6 |
| Q9Z126 | Cxcl4 | Chemokine (C-X-C motif) ligand 4 | Cytokine activity; chemotaxis | 2.07 | 0.03 | 3 |
| Q9EQI5 | Cxcl7 | Chemokine (C-X-C motif) ligand 7 | Cytokine activity; chemotaxis | 2.95 | 0.02 | 1 |
| P11276 | Fn1* | Fibronectin | Acute phase response; cell adhesion | 4.95 | – | 11 |
| P16110 | Lgals3 | Galectin-3 | Lectin; IgE binding | 2.84 | 0.04 | 4 |
| B1AQJ6 | Lgals3bp | Galectin-3 binding protein | Cell adhesion; integrin-signaling | 1.70 | 0.03 | 2 |
| Q6RUT2 | Mcp11 | Mast cell protease-11 | Peptidase activity | 2.54 | 0.02 | 3 |
| O08692 | Ngp | Neutrophilic granule protein | Defense response; peptidase inhibitor activity | 4.05 | 0.03 | 7 |
| P31725 | S100a9 | Protein S100-A9 | Bacterial carbohydrate sensing; antimicrobial | 3.22 | 0.01 | 4 |
| P07214 | Sparc* | SPARC | Cell growth; proliferation; Ca2+ | 14.79 | – | 1 |
| P21981 | Tgm2 | Transglutaminase-2 | GPCR signaling; cell adhesion; Ca2+ | 6.00 | 0.00 | 8 |
| Q8CIZ8 | Vwf* | von Willebrand factor | Focal adhesion; ECM-receptor interaction | 2.31 | – | 1 |
| O89098 | Cst7 | Cystatin-7 | Peptidase inhibitor activity | −3.96 | 0.01 | 2 |
| Q8BH61 | F13a | Coagulation factor XIII A chain | Blood coagulation; peptide cross-linking | −4.56 | 0.00 | 15 |
| P16045 | Lgals1 | Galectin-1 | Cell-cell adhesion; myoblast differentiation | −1.97 | 0.00 | 7 |
| B1AQR8 | Lgals9 | Galectin-9 | Lectin | −1.50 | 0.00 | 11 |
| Carbohydrate sensing | ||||||
| Q62230 | Cd169 | Sialoadhesin | Bacterial carbohydrate sensing; cell adhesion | 2.94 | 0.00 | 41 |
| Q61735 | Cd47 | Leukocyte surface antigen CD47 | Cell adhesion; response to bacterium | 1.84 | 0.01 | 4 |
| Q91V08 | Clec2d* | C-type lectin domain family 2 member D | Carbohydrate sensing; C-type lectin; differentiation | 2.30 | – | 3 |
| P01873 | Igh-6 | Ig mu chain C region membrane-bound form | Antigen binding; activation of MAPK activity | 9.95 | 0.03 | 13 |
| P19973 | Lsp1 | Lymphocyte-specific protein 1 | Chemotaxis; PKC signaling; leukocyte migration | 4.38 | 0.00 | 8 |
| Q61878 | Prg2 | Bone marrow proteoglycan | Carbohydrate sensing; C-type lectin; cytotoxin | 3.19 | 0.01 | 6 |
| Q9JL95 | Prg3* | Proteoglycan 3, Isoform 1 | Carbohydrate sensing; C-type lectin; cytotoxin | 7.68 | – | 1 |
| Q09200 | B4galnt1 | Beta-1,4 N-acetylgalactosaminyltransferase 1 | Bacterial carbohydrate sensing | −3.83 | 0.00 | 11 |
| A2AIV8 | Card9* | Caspase recruitment domain family, member 9 | C-type lectin coupling; NF-κB activation | −4.40 | – | 5 |
| A9XX86 | Clec10a* | Macrophage galactose-type C-type lectin 2 | Bacterial carbohydrate sensing | −3.11 | – | 1 |
| Q504P2 | Clec12a* | C-type lectin domain family 12 member A | Bacterial carbohydrate sensing | −3.55 | – | 3 |
| P09568 | Ly6c | Lymphocyte antigen 6C2 | −5.74 | 0.00 | 3 | |
| A9XX86 | Mgl2* | Macrophage galactose N-acetyl-galactosamine specific lectin 2 | Carbohydrate sensing; C-type lectin | −3.11 | – | 1 |
| Peptidases/peptidase inhibitors | ||||||
| P70202 | Lxn | Latexin | Metalloprotease inhibitor activity | 2.07 | 0.01 | 3 |
| Q9D154 | Serpinb1 | Serine proteinase inhibitor, clade B, member 1 | Peptidase inhibitor activity | 2.94 | 0.00 | 17 |
| Q3U3L3 | Serpinb6a | Serine proteinase inhibitor, clade B, member 6a | Peptidase inhibitor activity | 3.41 | 0.03 | 2 |
| O08804 | Serpinb6b* | Serine peptidase inhibitor, clade B, member 6b | Peptidase inhibitor activity | 1.96 | – | 2 |
| O08797 | Serpinb9* | Serine proteinase inhibitor, clade B, member 9 | Peptidase inhibitor activity | 4.95 | – | 6 |
| P19324 | Serpinh1 | Serine proteinase inhibitor, clade H, member 1 | Peptidase inhibitor activity | 3.12 | 0.04 | 5 |
| P28293 | Ctsg | Cathepsin G | Endopeptidase activity | −7.09 | 0.00 | 15 |
| Q3UP87 | Ela2 | Elastase-2 | Endopeptidase activity | −7.43 | 0.00 | 12 |
| Q61096 | Prtn3 | Proteinase 3 | Endopeptidase activity | −7.85 | 0.00 | 7 |
| Antiviral response | ||||||
| Q3U5Q7 | Cmpk2 | UMP-CMP kinase 2, mitochondrial; | LPS response; IFN response | 2.34 | 0.01 | 9 |
| Q9Z0E6 | Gbp2 | Interferon-induced guanylate-binding protein 2 | GTPase activity | 3.55 | 0.00 | 9 |
| Q80SU7 | Gvin1 | Interferon-induced very large GTPase 1 | GTPase activity | 3.45 | 0.00 | 34 |
| Q3U5K8 | Ifit1* | Interferon-induced tetratricopeptide repeats 1 | Viral ppp-RNA binding; IFIT complex | 3.24 | – | 4 |
| Q8R2S7 | Ifitm1* | Interferon induced transmembrane protein 1 | Control cell growth; anti-proliferative | 3.87 | – | 1 |
| Q9D8C4 | Ifi35 | Interferon-induced 35 kDa protein homolog | 1.47 | 0.03 | 5 | |
| Q61179 | Irf9* | Interferon regulatory factor 9 isoform 1 | Transcription regulation | 1.93 | – | 4 |
| Q0GUM2 | Irgb10* | Interferon-gamma-inducible p47 GTPase | Host resistance | 2.15 | – | 3 |
| Q60766 | Irgm1* | Immunity-related GTPase family M protein | Cell motility; adhesion; autophagy | 1.80 | – | 3 |
| Q64339 | Isg15* | Interferon-stimulated protein 15 | RLH signaling; Ubl conjugation pathway | 3.89 | – | 2 |
| Q9JL16 | Isg20* | Interferon-stimulated gene 20 kDa protein | DNA/RNA metabolism; exonuclease activity | 2.03 | – | 5 |
| B9EIU4 | Oas3 | 2'-5' oligoadenylate synthetase 3 | Viral RNA binding; OAS/RNase L pathway | 3.88 | 0.03 | 4 |
| Q60953 | Pml | Probable transcription factor PML | Antiviral defense; apoptosis; transcription regulation | 2.34 | 0.00 | 22 |
| Q6Q899 | RIG-I | Retinoic acid-inducible gene 1 protein | RLH signaling; viral RNA/DNA binding; | 4.16 | 0.00 | 17 |
| Q60710 | Samhd1 | SAM domain- and HD domain-containing 1 | Hydrolase activity | 2.67 | 0.00 | 15 |
| O35892 | Sp100 | Nuclear autoantigen Sp-100 | PML body; DNA binding | 2.10 | 0.05 | 8 |
| P42225 | Stat1 | Signal transducer and activator of transcription 1 | 1.79 | 0.01 | 15 | |
| P42227 | Stat3 | Signal transducer and activator of transcription 3 | 1.43 | 0.03 | 11 | |
| Q3T9E4 | Tgtp1 | T-cell specific GTPase 1 | Response to virus; GTPase activity | 3.16 | 0.00 | 10 |
| Q9DBK7 | Uba7 | Ubiquitin-activating enzyme E1-like | RLH signaling; ISG15 activating enzyme activity | 2.36 | 0.01 | 10 |
| Iron homeostasis | ||||||
| Q9JHI9 | Fpn1* | Ferroportin-1 | Iron ion transport; apoptosis | 2.15 | – | 2 |
| P09528 | Fth1 | Ferritin heavy chain | Iron ion storage | 1.75 | 0.01 | 7 |
| Q3THE6 | Ftl1 | Ferritin light chain 1 | Iron ion transport; oxidoreductase activity | 2.37 | 0.01 | 11 |
| Q60842 | Lcn2* | Lipocalin-2 | Iron ion transport;siderophore transport; apoptosis | 4.49 | – | 3 |
| P08071 | Ltf | Lactotransferrin | Iron ion transport; Peptidase activity; antimicrobial | 4.82 | 0.02 | 8 |
| Q8CI59 | Steap3 | Metalloreductase STEAP3 | Iron ion uptake; apoptosis | −2.47 | 0.03 | 6 |
| Q921I1 | Tf | Serotransferrin | Iron ion transport; cell proliferation | −1.73 | 0.02 | 24 |
| Q62351 | Tfrc | Transferrin receptor protein 1 | Iron ion uptake; osteoclast differentiation | −3.18 | 0.00 | 11 |
| ROS signaling | ||||||
| Q05117 | Acp5 | Tartrate-resistant acid ATPase | Cytokinesis; antibactericial; H2O2 repression | 5.26 | 0.02 | 1 |
| P03958 | Ada | Adenosine deaminase | Amplification ROS; adenosine deaminase activity | 2.07 | 0.00 | 11 |
| Q9EQ20 | Ald6a1 | Aldehyde dehydrogenase family 6 member A1 | Aldehyde dehydrogenase (NAD) activity; differentiation | 2.05 | 0.02 | 6 |
| Q3TFC7 | Ald7a1 | Aldehyde dehydrogenase family 7 member A1 | Aldehyde dehydrogenase (NAD) activity | 2.73 | 0.02 | 6 |
| Q64669 | Nqo1 | NAD(P)H dehydrogenase [quinone] 1 | Response to oxidative stress | 2.03 | 0.04 | 4 |
| P11247 | Mpo | Myeloperoxidase | Response to ROS; microbicidal activity; | −7.49 | 0.00 | 45 |
| Q3UBI5 | Ncf1 | Neutrophil NADPH oxidase factor 1 | Bacterial killing; superoxide-generating oxidase | −2.02 | 0.01 | 11 |
| O70145 | Ncf2 | Neutrophil NADPH oxidase factor 2 | Bacterial killing; superoxide-generating oxidase | −2.28 | 0.01 | 11 |
| Q3TBC6 | Ncf4 | Neutrophil NADPH oxidase factor 4 | Bacterial killing; superoxide-generating oxidase | −2.01 | 0.01 | 7 |
| ER stress response | ||||||
| P14211 | Calr | Calreticulin | Carbohydrate binding; Lectin; protein stabilization | −2.38 | 0.00 | 29 |
| Q91YW3 | Dnajc3 | DnaJ homolog subfamily C member 3 | Proteolysis | −2.01 | 0.00 | 12 |
| P20029 | Hspa5 | Heat shock 70 kDa protein 5 | Proteolysis | −2.16 | 0.00 | 43 |
| P08113 | Hsp90b1 | Heat shock protein 90kDa beta | Proteolysis; unfolded protein binding | −1.80 | 0.00 | 38 |
| Q99LP6 | Grpel1 | GrpE like protein 1 | Protein folding | −1.63 | 0.03 | 7 |
| Q9JKR6 | Hyou1 | Hypoxia up-regulated protein 1 | Response to hypoxia | −1.83 | 0.00 | 34 |
| Q921X9 | Pdia5 | Protein disulfide-isomerase A5 | Response to stress; cell redox homeostasis | −1.77 | 0.01 | 7 |
| Q922R8 | Pdia6 | Protein disulfide isomerase A6 | Cell redox homeostasis | −1.98 | 0.00 | 23 |