Fig. 1.

c-Src chemical rescue by imidazole. A, proposed R390A c-Src chemical rescue mechanism by imidazole indicating interactions of imidazole with the catalytic base Asp-388 and Tyr from the substrate (based on Protein Data Bank entry 3GEQ). B, comparison of the phosphotyrosine profile of imidazole-treated cells compared with that of untreated cells. Tyrosine phosphorylation was induced by imidazole treatment (10 mm, 5 min) of SYF cells stably transfected with c-Src R390A and D388N SYF (negative control). The cells were subjected to lysis, and whole lysates were subjected to Western blotting with 4G10 anti-phosphotyrosine antibody.